The Laboratory for Nonlinear Optical Microscopy is a bioimaging facility specialized in multi-photon fluorescence microscopy in-vivo. To learn more about our research activities and available Master-, PhD- or PostDoc positions please contact: albrecht.haase [at] unitn.it
The currently ongoing Research projects are:
Optical Tomography of Skin Lesions
We are developing non-invasive diagnostic methods for skin cancer. The focus is on the assessment of melanocytic lesions to distinguish malignant from begin lesions. The applied techniques are two-photon and second-harmonic-generation microscopy. Both techniques are particularly suited for this purpose due to their high spatial resolution and imaging depth and their low photo-damage, with respect to conventional optical imaging techniques. After studies of photo-damage limits in deep tissue imaging, a clinical study on suspicious nevi is performed.
Comparison: Histological H&E Gold standard and Two-photon auto-fluorescence image
- P. Bauer, Department of Dermatology, S. Chiara Hospital, Trento.
- S. Boi, Department of Pathology, S. Chiara Hospital, Trento.
The project is co-funded by the Provincià Autonoma di Trento, Bando di progetti per la ricerca sanitaria finalizzata 2009.
Sub-micron Containers for Drug Delivery
We produce sub-micron sized containers in diverse forms and compositions based on Calcium carbonate. Their synthesis is optimized regarding, form, size, and payload adsorption properties. The drug release dynamics is studied and its active control is one of the main goals of the project. The containers’ bio-compatibility and the penetration and transport dynamics in biological system are examined. Our drug-delivery systems stand out due to their simplicity and cost-effectiveness, their bio-compatibility and universal applicability.
Yellow: Dye-loaded Vaterite containers, Blue: recrystallized Calcite crystals after dye release
- Cells and Tissue Dynamics Lab of Antonella Motta at BIOtech, University of Trento
- T.V. Bukreeva, Shubnikov Institute of Crystallography of the Russian Academy of Science (Moskow)
- B.V. Parakhonskiy, A. Haase, and R. Antolini (2012). Sub-Micron Vaterite Containers: Synthesis, Substance Loading, and Release. Angewandte Chemie International Edition 51(5) 1195-97. doi:10.1002/anie.201104316
Il progetto è co-finanziato della Provincià Autonoma di Trento, Marie Curie Actions, Trentino COFUND 2009
Intra-cellular localization and trafficking of RNASET2
We study the cancer-antagonizing gene RNASET2 within cultured HeLa cells and the influence of the enzymatic functionality of the ribonuclease on the gene trafficking, since it was found to affect tumor growth. The applied method is Fluorescence Recovery after Photobleaching (FRAP) which allows comparing the intra-cellular dynamics of the wild-type gene and an activity deficient mutant.
Left) Sample images from an experimental sequence. Right) Averaged fluorescence recovery cuves
- L. Vidalino, L. Monti, A. Haase, A.M. Moro, F. Acquati, R. Taramelli, and P. Macchi (2012). Intracellular trafficking of RNASET2, a novel component of P-bodies. Biology of the Cell 104: 13-21. doi: 10.1111/boc.201100092